Abstract
Precursor feeding experiments establish that the isocyanide groups in xanthocillin-X monomethyl ether (XME)1 do not originate from (i) compounds associated with C1-tetrahydrofolate metabolism; (ii) the ureido group of citrulline, carbamoyl phosphate, or potassium cyanate; or (iii) cyanide and arguably related compounds. However the results with D-[U-13C] glucose show that the origin of the isocyanide groups is metabolically closer to glucose than is the origin of the backbone; DL-[1 -13C] glyceraldehyde and D-[1-13C]glucose are not specific precursors.
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