Abstract
A new culture system has been developed for production of protein. Mammalian cells, MKN 45, were cultured on the surface of a potential-controlled electrode. Proliferation rate of cells depended on the electrode potential. In the potential range above 0 V vs. AgAgCl, proliferation rate markedly decreased with an increase in electrode potential. Such a potential dependency of proliferation was reversible without any fatal effects in the potential range from 0 to 0.4 V vs. AgAgCl. These findings have made it possible to electrically regulate proliferation rate.Carcinoembryonic antigen (CEA) was produced by MKN 45 cells which were cultured on the surface of a potential-controlled electrode. The rate of CEA production was profoundly enhanced by a small potential application to the electrode. On the basis of these results an electrically controlled cell culture has been proposed.
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