Abstract
Abstract A new phosphoramidite-derived reagent, (2-cyanoethoxy)-2-(2′-0-4,4′- dimethoxytrityloxyethylsulfonyl)ethoxy-N,N-diisopropylaminophosphine, for the 5′- phosphorylatlon of ollgodeoxyribonucleotides has been developed. Phosphorylatlon efficiency can be determined by the release of 4,4′-dimethoxytrityl cation in acid.
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