Chemiluminescent optical fibre genosensor for porcine meat detection

Abstract

An easy, rapid and sensitive method for the detection of meat species is important to identify adulteration or fraudulent substitution in raw meats with undeclared species. We report herein the development of an optical fibre genosensor able to detect low level of pork (Sus Scrofa) meat in mixtures of minced meat obtained by an optimized and faster DNA extraction protocol. Our approach uses highly specific and sensitive DNA capture and secondary probes designed out of the mitochondrion genome of Sus Scrofa. Once DNA capture probes were immobilized on optical fibres, we demonstrate their putative application as genosensors in detecting Sus Scrofa DNA in purified samples obtained first using a commercial DNA extraction kit and thereafter using our improved extraction method. Furthermore, analysis of beef (Bos taurus) minced meat samples also show that the assay can determine 1% (w/w) of Sus Scrofa DNA. The sensitivity obtained by the optical fibre and the short time required for the results (about 2.5h) indicate the usefulness of the system. We envision that this genosensor may serve as a diagnostic tool for food sample screening using a technology that is accurate, simple to perform and has the potential to be used in portable format.