Chemiluminescence of the Ce(IV)/CDP-Star System Based on the Phosphatase-like Activity of Ce(IV) Ions.

Abstract

The phosphatase-like activity of Ce(IV) ions was applied for chemiluminescence (CL) analysis for the first time. Ce(IV) can catalyze the hydrolysis of CDP-star, which is a phosphatase substrate, to produce strong CL emission. The CL performance of the Ce(IV)/CDP-star system can be significantly improved by the addition of ionic liquids. In the presence of 1-butyl-3-methylimidazolium tetrafluoroborate, the selective and sensitive CL detection of Ce(IV) ions was achieved with a detection limit of 460 nM. The proposed CL system was also used for the detection of ascorbic acid and ClO–. It is based on the phenomenon that Ce(IV) can catalyze the hydrolysis of CDP-star, while Ce(III) cannot. The introduction of reductive ascorbic acid into the mixture of Ce(IV)/CDP-star can turn off the CL signal, while the addition of oxidative ClO– into the solution of Ce(III)/CDP-star can turn on the CL emission. Finally, Ce(IV)/CDP-star CL was successfully applied for evaluating the total antioxidant capacity in commercial fruit juice samples.