Abstract
The chemiluminescence generated following the reduction of flavin mononucleotide, flavin adenine dinucleotide and riboflavin by Fe(II) in pyrophosphate buffer is reported. These flavins also produce chemiluminescence in the presence of Fe(II) and hydrogen peroxide. The chemiluminescence generated from flavin mononucleotide, hydrogen peroxide and Fe(II) is five to six orders of magnitude lower than bacterial bioluminescence and in the absence of peroxide the chemiluminescence is reduced by a further order of magnitude. The role of hydroxyl radicals in the generation of electronic excited states is discussed and a possible mechanism proposed.
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Schedule a callMore From: Journal of Photochemistry & Photobiology, A: Chemistry
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