Abstract
Azo-para-phenylene-N-aza-15-crown-5)-phthalhydrazide presents weak fluorescence and (in the presence of aqueous phosphate buffer at pH = 10.1 with H2O2, with or without Fe 3+ or Cu 2+ ions) a significant chemiluminescence (CL) at λCL = 425 nm. Linear correlations exist between the hydrogen peroxide concentration and the intensity of the CL. For similarly intense chemiluminiscence, in the presence of cupric ions, the H2O2 concentration range is ten times lower than in their absence. This observation allows the determination of low concentrations of H2O2 and of Cu 2+ ions.
Highlights
The chemiluminescence (CL) of organic compounds[1] has both theoretical and practical interest
Phthalhydrazide derivatives have been prominent; especially luminol, (5-amino-2,3dihydrophthalazine-1,4-dione) has many uses owing to its fluorescence (FL) and chemiluminescence.[1,2,3,4,5,6,7]
It is possible to determine cholesterol via the H2O2 concentration formed by enzymatic oxidation.[14]
Summary
The chemiluminescence (CL) of organic compounds[1] has both theoretical and practical interest.
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