Chemiluminescence mechanisms of cerium-norfloxacin and its application in urine analysis

Abstract

AbstractIn this article, novel chemiluminescence mechanisms between norfloxacin and cerium(IV) in an acidic medium were studied. Chemiluminescence spectra of the present system were recorded observing three maximum emissions at about 475 nm, 550 nm, and 620 nm, respectively. The results indicate that the chemiluminescence peaks located at 475 nm and 620 nm can be ascribed to the emission of a singlet oxygen, while the chemiluminescence emission at 550 nm occurred in course of the reaction between acidic cerium(IV) and the phenolic intermediate. Under optimum conditions, the chemiluminescence intensity was linear with the concentration of norfloxacin over the range of 2.0 × 10−8−1.0 × 10−5 g mL−1 and the detection limit of 1.0 × 10−8 g mL−1 (S/N = 3). The relative standard deviation was 1.94 % for a 4.0 × 10−7 g mL−1 norfloxacin solution considering eleven repeated measurements. The present chemiluminescence system was successfully applied in the determination of norfloxacin in pharmaceutical preparations and concentration-time profiles in urine.