Chemiluminescence Determination of Sulpiride in Pharmaceutical Preparations and Biological Fluids Based on KMnO4‐Tween 80 Reaction

Abstract

AbstractChemiluminescence (CL) of an acidic KMnO4 system in the presence of Tween 80 was investigated for the determination of sulpiride. Strong CL was recorded when a mixture of sulpiride and Tween 80 was injected into acidic KMnO4 solution. The experimental parameters affecting the CL intensity were carefully studied using flow injection mode. Under the optimum conditions, the CL intensity was proportional to the concentration of sulpiride in the range of 5.0 × 10−7 ∼ 1.0 × 10−4 g mL−1. The detection limit is 1.6 × 10−7 g mL−1 sulpiride, and the relative standard deviation for 1.0 × 10−6 g mL−1 sulpiride solution is 1.5% (n = 11). The proposed method has been successfully applied to the determination of sulpiride in tablets and in spiked human plasma and urine samples.