Abstract
A new sulfonate reagent, 2-(2-naphthoxy)ethyl 2-[1-(4-benzyl)-piperazyl]ethanesulfonate, was synthesized for analytical derivatization in liquid chromatography. The reagent consists of two main moieties, i.e., one with a fluorophore (naphthoxy) for sensitive detection after being tagged to an analyte; and the other with a tertiary amino function (a substituted piperazine) that can be removed after derivatization by acid treatment. The reagent was applied to the derivatization of caproic acid as a model analyte. The resulting derivative was analyzed by high-performance liquid chromatography with fluorometric detection. The linear range of the method for the determination of caproic acid was over 0.01–0.5 nmol, with a detection limit (signal-to-noise ratio=3) of about 0.1 pmol. The excess reagent was readily removed from organic solvent after treatment with an aqueous acid solution.
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