Abstract
Monitoring the concentration of norepinephrine (NE), epinephrine (Ep) and dopamine-β-hydroxylase (DβH) is of great importance in studying and diagnosing related diseases. In this study, a simple chemically-modulated turn-on fluorescence strategy is proposed for rapid, selective and visual discrimination of trace NE and Ep. Herein, Ep is differentiated from other catecholamines via its fast fluorescence response in 1.0 M NaOH medium. Furthermore, NE is alkalized with 0.01 M NaOH and is then immediately triggered by polyethyleneimine to emit bright cyan fluorescence, whereas Ep and DA show no any fluorescence response. The fluorescence intensity of resultant solution is linearly proportional to the concentration of Ep and NE in the range of 50.0–10000.0 nM and 10.0–10000.0 nM, respectively. Moreover, NE and Ep as low as 500.0 nM can be fluorescently identified with naked-eye, which is convenient for rapidly preliminary diagnosis and self-test of hypercatecholaminism. Inspired by the catalytic property of DβH transforming DA into NE, the proposed strategy enables a simple and high-efficient fluorescence method to monitor DβH by using trace DA as substrate and directly quantifying NE. The method is simple, smart, highly efficient and eco-friendly, and has been applied to the rapid assay of NE, Ep and DβH in real samples.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
