Chemically Defined Medium for the Production of Phytase by Hanseniaspora guilliermondii S1, Pichia fermentans S2 and its Secondary Structure Prediction of 16S rRNA

Abstract

Chemical efficiency and medium formulation was the essential step for design highly demands laboratory experiments for the yield enhancement and productivity of phytase by two strains of Hanseniaspora guilliermondii S1 MG663578 and Pichia fermentans S2 MG663579.The SSF experimental model showed maximum phytase production of 365 U/ml appearing g/100ml: Phytic acid (1.5), peptone (0.15), dextrose (0.50), yeast extract (0.05), malt extract (0.05) pH 5.5 and 280C used 108 cells ml-1 culture Hanseniaspora guilliermondii S1 MG663578.In enzyme kinetics, Km and Vmax values were found to be 3.3 mM and 0.4 μmol/min using phytic acid as a substrate in Hanseniaspora guilliermondii S1 MG663578.The secondary RNA structure of active strain Hanseniaspora guilliermondii S1 MG663578 was predicted 15 stems in their MFE and Centroid secondary structure and dot-bracket notation showed that the free energy of the structure was - 165.7 kcal/mol; the threshold energy is -4.0 with cluster factor, conserved factor 2 and compensated factor 4; and the conservatively is 0.8.