Chemical variation within and among six northern willow species

Abstract

Plant tissues typically contain a diverse complement of secondary metabolites that serve as protection against various biotic and abiotic hazards. Chemical similarities are commonly used to infer phylogenetic relationships among plant taxa, but the studies are typically based on the mean concentration of each compound in each study species, thus overlooking within-species variability. In order to investigate patterns of intra- and interspecific chemical variation in plants, we measured the concentrations of condensed tannins and 36 other phenolic compounds in 120 leaf samples representing six northern Salix species. Multivariate clustering and ordination analyses of the data show that: (1) Despite considerable within-species variation in chemical profiles, intraspecific variability is on average lower than the variation among species. (2) Interspecific similarities are sensitive to the data analysis methods used, and different chemical classes produce partly contradictory results. (3) Compounds within each biosynthetic class tend to behave in a correlated manner and, consequently, overall chemical similarities are weakly correlated with the phylogeny of the studied species. The conclusion is that chemical data are poorly suited for phylogenetic inference, unless methods for data analysis are improved to take into account the biosynthetic routes by which the compounds are produced.