Abstract
Three step chemical tweaking of nonfluorescent parent GFP chromophore has yielded a three hundred times as bright coumarinic fluorophore. A fluorogenic compound has been prepared which after photo-uncaging yields the same highly bright fluorophore that could be used for live stem cell imaging.
Highlights
Three step chemical tweaking of nonfluorescent parent GFP chromophore has yielded a three hundred times as bright coumarinic fluorophore
Different chemical modi cations/substitutions in benzene and imidazole ring of p-HBDI have been tried but so far a maximum of only $10% uorescence quantum yield could be achieved in GFP chromophore analogues.[10]
There are quite a few number of photoactivable compounds, there is only one report so far where a photoactivable compound has been made with GFP chromophore analogue.[13]
Summary
Three step chemical tweaking of nonfluorescent parent GFP chromophore has yielded a three hundred times as bright coumarinic fluorophore. The quest to enhance the uorescence quantum yield of non uorescent GFP (green uorescent protein)[1,2] chromophores (p-HBDI, see Scheme 1) has engaged many researchers from biology and chemistry for more than a decade. In this manuscript we describe how three step chemical tweaking of non- uorescent parent GFP chromophore (p-HBDI) could lead to a highly uorescent coumarinic uorophore (Scheme 1).
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