Abstract
Summary Samples of disodium phenyl phosphate substrate were heat treated or autoclaved and stored with unheated and chloroform-preserved samples at 5 and 20° C. for 1 yr. Aliquots were removed from storage at regular intervals for estimation of free phenol, using N, 2, 6-trichloro- p -quinoneimine (CQC) as indicator. Free phenol was found in all unheated samples after 2 wk. of storage. No free phenol was found in any of the heat-treated or autoclaved samples after 1 yr. of storage. The heat treatment and age of the buffer-substrate had no effect on the sensitivity of the phosphatase method.
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