Chemical priming with urea and KNO3 enhances maize hybrids (Zea mays L.) seed viability under abiotic stress

Abstract

Seed priming is a method to improve germination and seedling establishment under stress conditions. The effect of seed priming in chemical solutions such as urea and KNO3, on protein and proline content, germination, and seedling growth responses of four maize (Zea mays L.) hybrids under drought and salt stress conditions was studied in a controlled environment in 2010. Treatments included stress type and intensity at five levels: moderate drought (MD), severe drought (SD), moderate salt (MS), severe salt (SS), and control (C1, without stress), three seed priming types including water (C2, as control), KNO3, and urea (as chemical priming), and four maize hybrids including Maxima, SC704, Zola, and 307. The results showed that the highest germination percentage (Ger %), germination rate (GR), seedling length (SL), radical length (RL), and seedling to radical length ratio (S/R) were achieved in no stress treatments and most proline content in SD treatment. Urea priming led to more Ger%, GR, and SL compared to other primers and treatment under KNO3 priming resulted in higher RL compared to other primers. Chemical priming had no effect on S/R and proline content. Also, in terms of most traits, no difference was found among the four hybrids. Results showed that salt stress could affect GR and RL more than the drought stress. Drought stress affected germination percentage and S/R more than the salt stress. Both stresses decreased all measured parameters, except protein and proline content which were increased remarkably, and more under drought compared to salt stress. Based on proline content, hybrid 304 appeared to be more resistant to stress than other hybrids. Generally, KNO3 and urea alleviated effects of both stresses and led to increased germination and seedling growth as well as the root length. Therefore, priming could be recommended for enhancing maize growth responses under stressful conditions.