Chemical physical characteristics of Bombyx mori L C301 pupae under heat shock treatment

Abstract

Bombyx mori pupae, the remaining of silk thread spinning contains a fairly high protein. Protein is not resistant to heat temperatures. Heat shock application causes failure of pupae formation and will affect the content of pupae organic compounds. Heat shock was given for 3 hours at 42°C when the larvae in instar IV, then the larvae were rearing until they become cocoons. Pupae was removed from the cocoon, then pupae morphology was observed. Analysis of pupae sampling includes protein, total fat and carbohydrates based on SNI 01-2891-1992.The failure of pupae formation was indicated by the blackish brown pupae and the irregular shape of the cocoon, while the pupae without heat shock was light brown.The content of silkworm pupae organic compounds that were given a heat shock was 80.4% protein.Total fat was 2.13% and carbohydrates were 6.58%. Heat shock did not affect the level of protein in pupae.