Chemical Modification of Peptides and Proteins Using Spirooxindole Oxirane Derivatives

Abstract

AbstractMethods for chemical modification of proteins and peptides at certain positions are required for the preparation of antibody‐drug conjugates, protein‐ or peptide‐biotin conjugates, and conjugates used as therapeutics or as tools in biomedical research. We have developed chemical modification methods that use spirooxindole oxirane derivatives for the synthesis of protein‐ and peptide‐conjugates. In most cases, the epoxide group of the spirooxindole oxirane derivatives reacted to form a covalent bond with certain histidine residues and/or the N‐terminal amino group of peptides and proteins. The modification reactions of various proteins resulted in the formation of proteins modified at only one or two positions.