Abstract
1. Several modifiers capable of modifying hemoglabin in vitro have been used for in vivo studies in rats and rabbits under various conditions. 2. The results confirm our previous findings of in vitro studies that Schiff-base formation and glycosylation are reversible processes, whereas acylation produces irreversible modification. 3. The relative survival time of red cells with modified hemoglobin in comparison to the normal control samples was studied by double labelling technique with radioactive leucines. 4. Red cell morphology and fragility were also studied on these samples. No apparent abnormalities were observed.
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