Abstract
Phosphorylation is a key event in many cellular processes like cell cycle, transformation of environmental signals to transcriptional activation or polar growth. The chemical genetics approach can be used to analyse the effect of highly specific inhibition in vivo and is a promising method to screen for kinase targets. We have used this approach to study the role of the germinal centre kinase Don3 during the cell division in the phytopathogenic fungus Ustilago maydis. Due to the easy determination of the don3 phenotype we have chosen this approach for a genetic course for M.Sc. students and for IMPRS (International Max-Planck research school) students. According to the principle of “problem-based learning” the aim of this two-week course is to transfer knowledge about the broad spectrum of kinases to the students and that the students acquire the ability to design their own analog-sensitive kinase of interest. In addition to these training goals, we benefit from these annual courses the synthesis of basic constructs for genetic modification of several kinases in our model system U. maydis.
Highlights
The Bologna Declaration in 1999 started a process for the harmonisation of the architecture of the higher education system in Europe [1]
“problem-based learning” the aim of this two-week course is to transfer knowledge about the broad spectrum of kinases to the students and that the students acquire the ability to design their own analog-sensitive kinase of interest. In addition to these training goals, we benefit from these annual courses the synthesis of basic constructs for genetic modification of several kinases in our model system U. maydis
For transformation of ligation products in E. coli we propose the use of student-made electrocompetent cells
Summary
The Bologna Declaration in 1999 started a process for the harmonisation of the architecture of the higher education system in Europe [1]. The aim of this process was to set up three cycles with the qualifications Bachelor’s degree, Master’s degree and Doctoral degree to ensure compatibility in the standards and the quality of higher education in Europe. For the higher education system in Germany this declaration. At the Philipps-University of Marburg the “Biology” Bachelor-cycle was started in 2004 and the “Molecular and Cellular Biology” Master-cycle in 2007. As part of the Master module “Molecular characterisation of genes” the practical course “Chemical genetics” was created in 2009
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