Chemical Fingerprint Analysis and Quantitative Analysis of Saccharides in Morindae Officinalis Radix by HPLC-ELSD.

Hongmei Sun,Yini Cai,Enyao Ma,Xinjun Xu,Jie Shen,Zhimin Zhao,Depo Yang,Xiuwei Yang

doi:10.3390/molecules26237242

Abstract

A method based on high performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) was developed for the quantitative analysis of three active compounds and chemical fingerprint analyses of saccharides in Morindae officinalis radix. Ten batches of Morindae officinalis radix were collected from different plantations in the Guangdong region of China and used to establish the fingerprint. The samples were separated with a COSMOIL Sugar-D column (4.6 mm × 250 mm, 5 μm) by using gradient elution with water (A) and acetonitrile (B). In addition, Trapped-Ion-Mobility (tims) Time-Of-Flight (tims TOF) was used to identify saccharides of Morindae officinalis radix. Fingerprint chromatogram presented 26 common characteristic peaks in the roots of Morinda officinalis How, and the similarities were more than 0.926. In quantitative analysis, the three compounds showed good regression (r = 0.9995–0.9998) within the test ranges, and the recoveries of the method were in the range of 96.7–101.7%. The contents of sucrose, kestose and nystose in all samples were determined as 1.21–7.92%, 1.02–3.37%, and 2.38–6.55%, respectively. The developed HPLC fingerprint method is reliable and was validated for the quality control and identification of Morindae officinalis radix and can be successfully used to assess the quality of Morindae officinalis radix.

Highlights

Morinda officinalis How (Rubiaceae) is one of the most popular medicinal plants in southern regions in China, such as Guangdong, Fujian and Guangxi provinces [1]
The saccharides were extracted via 60% ethanol through heating reflux, avoiding the effect of low pH that led to the degradation of polysaccharides
A powerful and reliable high performance liquid chromatography (HPLC)-evaporative light scattering detection (ELSD) method after validation using a new-type sugar-based column was developed for the quality assessment of the roots of Morinda officinalis How for the first time

Summary

Introduction

Morinda officinalis How (Rubiaceae) is one of the most popular medicinal plants in southern regions in China, such as Guangdong, Fujian and Guangxi provinces [1]. Guangdong is the main producing area of the genuine medicinal materials of Morindae officinalis radix. Morindae officinalis radix is mainly used for the treatment of impotence, osteoporosis, depression, and inflammation [2,3,4]. Oligosaccharides, polysaccharides, iridoids, and anthraquinones are the main active components of Morindae officinalis radix [5]. In 2012, fructooligosaccharides (DP3DP9) of Morindae officinalis radix received permission to be prescribed for the treatment of depression from the Chinese Food and Drug Administration [14]

Methods

Results

Conclusion