Abstract
An ion selective electrode was used to monitor binding of Cd2+ on two bacteria, Bacillus subtilis (Gram+) and Escherichia coli (Gram−), as a function of increasing pH. A competitive Langmuir sorption isotherm was used in conjunction with a linear programming method (LPM) or FITEQL to fit experimental data. Results obtained with simulated data showed that LPM is less sensitive than FITEQL to variations in sorption data. Application of the LPM to experimental data found three discrete metal binding sites on B. subtilis and E. coli with −log equilibrium constant (pKS) values of −0.80±0.20, 0.63±0.09, and 2.35±0.10, and −0.60±0.10, 0.25±0.19, and 1.93±0.17, respectively, at a constant ionic strength, I=0.1 M (KNO3). The corresponding site densities were 0.09±0.01, 0.07±0.01, and 0.07±0.01, and 0.01±0.002, 0.02±0.01, and 0.04±0.01 μmol of Cd2+/mg of B. subtilis or E. coli. From FITEQL, pKS values of −1.18±0.15, 0.40±0.11, and 2.31±0.32 for B. subtilis and −1.46±0.34, 0.20±0.12, and 1.87±0.12 for E. coli were recovered with site densities of 0.10±0.07, 0.07±0.06, and 0.06±0.02, and 0.02±0.005, 0.02±0.004, and 0.04±0.04 μmol of Cd2+/mg of B. subtilis or E. coli, respectively. Total site densities of 0.22±0.02 and 0.06±0.01 μmol/mg were obtained by LPM for B. subtilis and E. coli, whereas FITEQL yielded values of 0.23±0.02 and 0.08±0.07 μmol/mg. Both LPM and FITEQL produced feasible results, but LPM was less sensitive to error and did not require an a priori assumption of the number of binding sites.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have