Abstract
Polygonatum sibiricum is traditionally used as Chinese medicine for immunity enhancement. Exploration of polysaccharides from Polygonatum species would provide a wider insight into the studies on their chemical structures and function activities. In this study, the alkali-extracted polysaccharide from P. sibiricum (PSP) was isolated and examined. The polysaccharide was firstly isolated by ion-exchange chromatography equipped with DE52 column, followed by isolated through Superdex-200 column. The obtained PSPJWA fraction was a homogenous one with average molecular weight of 141 kDa. The monosaccharide composition was galactose, arabinose and rhamnose in a ratio of 14:4:1. The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis. NMR and enzymatic studies showed that PSPJWA was a novel arabinogalactan-type structure. PSPJW polysaccharides with different molecular weight and similar chemical structure had different antioxidant activities. Together, P. sibiricum polysaccharide has the potential to be a natural antioxidant.
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