Abstract
Silk fibroin (SF), chitosan (C), and gelatin (G) blend composition in formic acid have been manufactured by using electrospinning technique in our previous work. The SF: G: C at weight ratios of 10: 20: 1 and 20: 10: 1 (%wt: %wt: ml) were used for crosslinking testing. Glutaraldehyde (GA), and 1-ethyl-3-(3-dimethylaminopropyl) cabodiimide (EDC) / N-hydroxy succcinimide (NHS) (EDC/NHS) were chose as crosslinking agents. All samples were treated in such agents by fumigation for 72 hours followed by dipping for 10 minutes. Then those samples were washed in distill water for 3 times (1 time per 10 minutes) and dried in desiccator at room temperature. GA caused immediately shrink in both nanofiber mats and became clearly visible yellowish. However, a little shrinkage occurred after dipping in EDC/NHS. It possibly concluded that the EDC / NHS appropriated for SF: G: C blend nanofiber mats.
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