Chemical Constituents of the Leaves of Campanula takesimana (Korean Bellflower) and Their Inhibitory Effects on LPS-induced PGE2 Production.

Yutong Qi,Hee-Soo Han,Kyung-Tae Lee,Se-In Choi,Hak Cheol Kwon,Hye Shin Ahn,Dae Sik Jang,So-Ri Son,Yu-Kyong Shin,Sun Hee Lee

doi:10.3390/plants9091232

Abstract

Campanula takesimana Nakai (Campanulaceae; Korean bellflower) is one of the endemic herbs of Korea. The plant has been used as traditional medicines for treating asthma, tonsillitis, and sore throat in Korea. A hot water extract of the leaves of C. takesimana exhibited a significant inhibitory effect on lipopolysaccharide (LPS)-stimulated prostaglandin E2 (PGE2) production. Repetitive chromatographic separation of the hot water extract led to the isolation of three new neolignan glucosides, campanulalignans A–C (1–3), with 15 known compounds (4–18). The structures of new compounds 1–3 were elucidated by analyzing nuclear magnetic resonance (NMR) spectroscopic data, along with high resolution quadrupole time of flight mass (HR-Q-TOF-MS) spectrometric data. Among the isolates, simplidin (7), 5-hydroxyconiferaldehyde (11), icariside F2 (12), benzyl-α-l-arabinopyranosyl-(1″→6′)-β-d-glucopyranoside (13), and kaempferol 3-O-β-d-apiosyl (1→2)-β-d-glucopyranoside (15) were isolated from the Campanulaceae family for the first time. The isolates (1, 2, and 4–18) were assessed for their anti-inflammatory effects on LPS-stimulated PGE2 production on RAW 264.7 cells. 7R,8S-Dihydrodehydrodiconiferyl alcohol (5), 3′,4-O-dimethylcedrusin 9-O-β-glucopyranoside (6), pinoresinol di-O-β-d-glucoside (8), ferulic acid (10), 5-hydroxyconiferaldehyde (11), and quercetin (18) showed significant inhibitory effects on LPS-stimulated PGE2 production.

Highlights

Campanula takesimana Nakai (Campanulaceae), known as Korean bellflower, is a native herb ofKorea, growing on Ulleng island
We describe in this paper the isolation metabolites from the leaves of P. japonicus, structure elucidation of the three new neolignan glucosides of the secondary metabolites from the leaves of P. japonicus, structure elucidation of the three new
The leaves of Campanula takesimana Nakai (Campanulaceae; Korean bellflower) used in present study were provided by COSMAX BIO, Gyeonggi-do, Republic of Korea, in July 2019

Summary

Introduction

Campanula takesimana Nakai (Campanulaceae), known as Korean bellflower, is a native herb ofKorea, growing on Ulleng island. Campanula takesimana Nakai (Campanulaceae), known as Korean bellflower, is a native herb of. The leaves and stems of C. takesimana are shiny, and the flowers have spots on a pale purple background. It is clearly distinguished from C. punctata, commonly distributed in Korea, China, and Japan, by its smaller overall size and lighter purple color of flower [1]. Because it has long flowering period and shape of flower, this plant has been commonly cultivated for a decorative plant. Besides the value of horticulture, roots of C. takesimana have been used for herbal remedies for asthma, tonsillitis, and sore throat in traditional Korean medicine [2].

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