Abstract
Anti-inflammatory compounds were investigated from the ethanol extract of the roots and rhizomes of Asarum heterotropoides var. mandshuricum, a traditional Chinese medicine called Xixin and used for pain and inflammatory. Nine new compounds were isolated, including six new lignans, neoasarinin A–C (1–3), neoasarininoside A and B (4 and 5), and asarinin B (7), and one new monoterpene, asarincin A (8), two new amides, asaramid II and III (10 and 11), and one new natural monoterpene, asaricin B (9), along with 37 known compounds (6, 12–47). Their structures and absolute configurations were elucidated on the basis of spectroscopic methods and chemical analyses. This is the first report of the absolute configuration of asarinin A (6). The 8-O-4′ neolignans (1–5) were reported in the genus Asarum for the first time. The 15 compounds 17, 19, 22–25, 28, 31, 36, 40, 42, 43, 45–47 were isolated from the genus Asarum, and compounds 16, 32, 33, 37 and 39 were isolated from A. heterotropoides var. mandshuricum for the first time. Thirty-seven of the isolates were evaluated for anti-inflammatory activity against the release of β-glucuronidase in polymorphonuclear leukocytes (PMNs) induced by the platelet-activating factor (PAF), and compounds 1, 4, 7, 8, 14, 17–19, 22, 24, 25, 29, 30, 32, 33, 40–43, 45, and 46 showed potent anti-inflammatory activities in vitro, with 27.9%–72.6% inhibitions at 10−5 mol/L. The results of anti-inflammatory assay suggested that lignans obtained from the CHCl3 extract might be the main active components of Xixin.
Highlights
The roots and rhizomes of Asarum heterotropoides Fr
The EtOH extract of the roots and rhizomes of A. heterotropoides var. mandshuricum was suspended in H2 O and partitioned with petroleum ether, CHCl3, EtOAc, and n-BuOH, successively
The roots and rhizomes of A. heterotropoides var. mandshuricum are used in traditional Chinese medicine for the treatment of pain and inflammatory diseases [1]
Summary
The roots and rhizomes of Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag, which is described as Xixin in Chinese or Asari Radix et Rhizoma (AR) in the Pharmacopoeia of the People’s Republic of China (2010 edition), is one of the most important traditional Chinese medicines of the genus Asarum (Aristolochiaceae). Mandshuricum (Maxim.) Kitag, which is described as Xixin in Chinese or Asari Radix et Rhizoma (AR) in the Pharmacopoeia of the People’s Republic of China (2010 edition), is one of the most important traditional Chinese medicines of the genus Asarum (Aristolochiaceae) It was widely used in traditional medicine as an anodyne and anti-allergic remedy to treat various diseases such as aphthous stomatitis, headache, toothache, and inflammation in some countries of Asia [1]. In order to evaluate the anti-inflammatory activity of non-volatile constituents, 37 compounds were screened in vitro by assessing the inhibitory rate of the release of β-glucuronidase in polymorphonuclear leukocytes (PMNs) induced by the platelet-activating factor (PAF) in this paper
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.