Abstract
In the present study, the chemical investigation of the leaves of Annona reticulata has resulted in the identification of nine compounds, including annonaretin A, (1), a new triterpenoid. The purified compounds were subjected to the examination of their effects on NO inhibition in LPS-activated mouse peritoneal macrophages and most of them exhibited significant NO inhibition, with IC50 values in the range of 48.6 ± 1.2 and 99.8 ± 0.4 μM.
Highlights
The Annona genus (Annonaceae) consists of about 119 species, most of which are shrubs and trees widely distributed in the tropical and subtropical regions, including the Southeast Asia countries such as Malaysia, Indonesia, Thailand, Cambodia, Laos, and Vietnam
Air-dried and powdered leaves of A. reticulata L. were soaked with methanol at room temperature, and the combined extracts were concentrated to give a deep brown syrup
The crude extract was suspended into water and partitioned with n-hexane, ethyl acetate, and n-butanol, successively to afford n-hexane, ethyl acetate, n-butanol, and water solubles, respectively
Summary
The Annona genus (Annonaceae) consists of about 119 species, most of which are shrubs and trees widely distributed in the tropical and subtropical regions, including the Southeast Asia countries such as Malaysia, Indonesia, Thailand, Cambodia, Laos, and Vietnam. In Indian folk medicine, various species of Annona have been used as vermifuges, anti-inflammatory agents, in wound healing, as antimalarial agents and in the treatment of diarrhoea and dysentery [1]. Reticulatacin and kaurane diterpenes were identified from the bark of the plant [7]. Other terpenes such as spathenelol, muurolene, copaene and eudesmol were reported in the previous literature [8]. In our continued program aimed at the identification of anti-inflammatory drug leads from natural sources, the chemical composition of the leaves of A. reticulata was investigated to search for the bioactive constituents by assays of inhibitory effects on NO inhibition in LPS-activated mouse peritoneal macrophages. We wished to report the characterization of nine compounds, including the structural determination of new compound 1, as well as their NO inhibitory effects
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