Chemical Constituents from Euphorbia kansui.

Qiao Zhang,Pei-Dong Chen,Jian-Wei Lou,Qin-Rong Zhou,Wei-Feng Yao,Wei-Wei Tao,Li Zhang,Yu-Ping Tang,Kun Wang,Guan-Cheng Dai

doi:10.3390/molecules22122176

Abstract

In this research, a new triterpenoid, tirucalla-8,24-diene-3β,11β-diol-7-one (1), and eupha-8,24-diene-3β,11β-diol-7-one (2), which was isolated from Euphorbia kansui for the first time, together with twelve other known compounds (3–14), were isolated from the ethyl acetate extract of Euphorbia kansui. Their structures were elucidated based on High resolution electrospray ionization mass spectrometry (HR-ESI-MS), Infrared Spectroscopy (IR), 1D and 2D Nuclear Magnetic Resonance (NMR) data. Both constituents 1 and 2 exhibited moderate cytotoxicity against colon cancer HCT-116, gastric cancer MKN-45 and breast cancer MCF-7.

Highlights

The plants of Euphorbia contain more than 2000 species spread all over the world, and about80 species distribute in China [1,2]
HPLC: Hanbon NP 7000 (Jiangsu Hanbang Technology Companies, Huaian, China) Serials pump with an NU 3000 Serials UV-Vis detector (Jiangsu Hanbang Technology Companies, Huaian, China), Phecda Si (20 × 250 mm, 5 μm); Waters 1525 with a 2996 Diode Array Detector (DAD) (Waters, Milford, CT, USA), XBrige-Prep C18 (150 × 19 mm, 5 μm), (Ultimate XB-C8, 30 × 150 mm, 5 μm)
The dried root of Euphorbia kansui was collected from Red River valley of Baoji, Shaanxi

Summary

Introduction

The plants of Euphorbia contain more than 2000 species spread all over the world, and about. 80 species distribute in China [1,2]. The structure type of the compounds in Euphorbia kansui are diterpenes, triterpenes, flavonoids, phenolic and acids [3,4,5]. Diterpenes and triterpenoids are the main compounds in Euphorbia kansui, which show a wide range of pharmacological activities, such as antiviral, skin irritating and modulation of multidrug resistance effects [6,7,8,9]. A new tirucallane-type triterpene named tirucalla-8,24-diene-3β,11β-diol-7-one (1) was first isolated from natural plants, and an euphane-type triterpene named eupha-8,24-diene-3β,11β-diol-7-one (2) (Figure 1) was isolated from Euphorbia kansui for the first time in our present study.

General Experimental Procedures

Plant Materials

Extraction and Isolation

Cytotoxicity Test

Conclusions