Abstract
Amygdalus persica L., belongs to Rosaceae family, and its flowers are used as medicine and food. n-Butanol extract of A. persica flowers were isolated and purified with various column chromatographies, and the fourteen compounds, chlorogenic acid butyl ester (1), rutin (2), protocatechuic acid (3), caffeic acid (4), 5-O-coumarroylquinic acid methyl ester (5), kaempferol-3-O-neohesperidoside (6), quercetin-3-O-β-D-glucoside (7), 3,5-dicaffeoylquinic acid (8), quercetin-3-O-α-L-rhamnoside (9), 5-O-coumaroylquinic acid (10), kaempferol-3-O-α-L-rhamnoside (11), kaempferol-3-O-β-D-galactoside (12), D-glucitol (13), and multiflorin A (14), were identified by spectroscopic data and physical data. All the compounds except compound 2 were identified from A. persica flowers for the first time. The compounds were investigated for their coagulation activity by activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT), and bibrinogen (FIB) in vitro. The results of coagulation activity showed that rutin (2), caffeic acid (4), kaempferol-3-O-neohesperidoside (6), kaempferol-3-O-α-L-rhamnoside (11), and kaempferol-3-O-β-D-galactoside (12) exhibited significant procoagulant activity, while chlorogenic acid butyl ester (1) possessed anticoagulant activity in vitro.
Highlights
Amygdalus persica L., belonging to Rosaceae, is an excellent plant resource that had the concomitant function of both medicine and foodstuff, and with relaxing bowel, diuresis, and reducing swelling effects [1]
Zeng et al analyzed the conditions for purification of total flavonoids from A. persica by macroporous resin, with adsorption-desorption ratios as indexes, and optimum resin DM-28 was selected by static adsorption-desorption and dynamic adsorption-desorption ratio tests and found that flavonoids had an obvious antioxidant activity. e IC50 values of DPPH and ABTS were 13.47 μg/mL and 32.16 μg/mL, respectively [6]
We found that the constituents of ethyl acetate extract from A. persica flowers showed strong coagulation activity
Summary
Amygdalus persica L., belonging to Rosaceae, is an excellent plant resource that had the concomitant function of both medicine and foodstuff, and with relaxing bowel, diuresis, and reducing swelling effects [1]. A. persica flowers are widely used in traditional Chinese medicine and used as a healthy food for beauty and constipation treatment, as well as wine and tea drinks [2, 3]. There are few studies on chemical composition and pharmacological activity of A. persica, mainly focusing on the volatile constituents and extraction process of polyphenols. Yuan et al studied the optimum ultrasonic-assisted extraction technology of polyphenols from A. persica with response surface methodology and found the polyphenols had certain scavenging effects on hydroxyl and DPPH radicals [4]. Zhang et al found that the major volatile constituents of A. persica flowers were linolenic alcohol, n-hexadecanoic acid, cyclohexane, and octadecanoic acid [5]. Zeng et al analyzed the conditions for purification of total flavonoids from A. persica by macroporous resin, with adsorption-desorption ratios as indexes, and optimum resin DM-28 was selected by static adsorption-desorption and dynamic adsorption-desorption ratio tests and found that flavonoids had an obvious antioxidant activity. e IC50 values of DPPH and ABTS were 13.47 μg/mL and 32.16 μg/mL, respectively [6]
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