Chemical compositions and larvicidal effectiveness of Rhododendron ponticum essential oil on Neodiprion sertifer larvae

Abstract

In many countries throughout the world, the European pine sawfly, Neodiprion sertifer (Geoffroy, 1785) (Hymenoptera: Diprionidae) is known as a significant forest pest that damages pine needles. As a result of the insect damage, young pine trees, especially, might dry up totally. Mechanical, biotechnical, biological, and chemical approaches are applied in research to keep it under control. Due to the increasing danger of chemical contamination associated with the use of synthetic pesticides to control insects, alternative control strategies have been sought, and studies on essential oils have been prioritized. In this research, in addition to determining the chemical analysis of Rhododendron ponticum L. (Ericales; Ericaceae) essential oil by GC–MS, the larvicidal effects of R. ponticum on N. setifer in the six larval stages were also investigated. The research was conducted in the Artvin Çoruh University Central Laboratory under laboratory settings (25 ± 1 °C, 65 ± 5% RH, and 14L:10D h photoperiods) during 2020–21. 1-Methyl-2-pyrrolidone (38.41%), Phenylethy alcohol (10.07%), Linalool (8.01%), Myrtenol 5.68%), β-Pinene (4.26%), Citronellol (4.05%), α-Pinene (3.02%) were found to be the major components of R. ponticum oil. The administration of R. ponticum essential oil at 10, 15, and 20 μL/Petri doses, as well as controls at the 24th, 48th, 72nd, and 96th hours, produced various rates of death (19.6–100%) on six larval stages of N. sertifer. According to LD50 and LD90 values, the highest toxicity was determined as L1 (0.58 μL/larva) and the lowest toxicity was as L6 (10.44 μL/larva). In conclusion, R. ponticum essential oil showed high toxicity against L1 and L2 larval stages of N. sertifer.