Abstract
Three volatile fractions (E1, E2, and E3) derived from the volatile extract of the aerial portion of Echiochilon fruticosum Desf. growing in Tunisia were analysed by GC and GC-MS. Fourty-nine different volatile compounds belonging to various families were identified in the previously indicated fractions. The main compounds were octadeca-9,12-dienoic acid (29.52, 32.24 %; in E2 and E3 respectively), carvacrol (17.52 %; in E1), β-cayophyllene (12.57 %; in E2) and abietatriene (17.82 %; in E1). Growth inhibition, antifeedant and toxicity effects of the crude volatile extract and its three fractions were examined against adults and larvae of Tribolium confusum du Val. The crude volatile extract and all fractions show significant inhibition of larval growth and antifeedant activity against adults of Tribolium confusum. However, fraction E1 seemed to be attractive to the larvae. The mortality of larvae kept on E1 volatile extract fraction reached 100% by the 12th day of treatment.
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