Chemical Composition of the Volatile Oils from the Aerial Parts of Artemisiaannua L. (Asteraceae) by Using Head Space Solid Phase Microextractionand Hydrodistillation Methods Prior to Gas Chromatographic-MassSpectrometric Determination: A Comparative Investigation

Abstract

The present report deals with the analysis, comparison of content and composition of the volatiles obtained by hydrodistillation (HD) and head space-solid phase microextraction (HS-PME) procedures from the fresh aerial parts of Artemisia annua L. The SPME headspace volatiles were collected using a 100 μm polydimethylsiloxane (PDMS) fiber. The composition of the volatile essential oils were investigated by GC-FID and GC-MS. Two series of natural compounds were identified constituting 94.6 and 95.18 % of the oils for HD and HS-SPME methods. In the water distilled oil, camphor (16.30 %), β-selinene (10.41 %), artemisia ketone (8.79 %), germacrene-D (7.14 %), α-pinene (4.79 %), 1,8-cineol (4.38 %) and α-selinene (4.09 %) were found to be major components. Moreover, the oil extracted by HS-SPME technique was mainly composed of α-pinene (12.03 %), artemisia ketone (11.24 %), β-selinene (8.73 %), 1,8-cineol (6.76 %), camphene (4.90 %) and α-selinene (4.85 %) as the most abundant fractions. Results imply that the use of HS-SPME could somewhat affect the composition of the essential oils