Chemical Composition of the Low-Polar Fraction of the Copitarsia uncilata Burgos & Leiva (Lepidoptera: Noctuidae) Eversible Pheromone Gland.

Abstract

In Colombia, Copitarsia uncilata Burgos & Leiva has been reported as a pest of ornamental flowers. The leafworm causes serious economic losses to farmers because the shipments are intercepted at the detection sites. The identification and subsequent synthesis of the sex pheromone compounds can lead to the use of semiochemicals as an important strategy to complement the pest management. This paper identifies and characterizes the chemical components of the female sexual gland of C. uncilata at three different age stages in order to relate the effect of age on the gland extract composition. Eggs and larvae were collected from commercial crops. The specimens were reared and sexed. Pheromone glands of intact females were excised at (a) 1-2, (b) 3-4, and (c) 5-6days old. The extracts were analyzed using GC-MS after two extraction time points: 20min and 8days. The extracts from the third age stage showed an increase in concentration for 35% of the compounds after 8days of storage. The relative abundance as a function of the female age showed two trends: an upward trend, where the relative abundances of the compounds increased through the age stages, while some compounds shown a downward trend. Within the first trend, mostly of the compounds were found to be related with infochemicals type structures, where tetradec-9-en-1-yl acetate (within several C-14 long-chain alcohols) is the main one. The 9-14:Ac quadrupled concentration through the ages is possibly related to the increased length of calling period as female moth age.