Chemical Composition of the Essential Oils and Volatiles of Salvia leriifolia by Three Different Extraction Methods Prior to Gas Chromatographic-Mass Spectrometric Determination: Comparison of HD with SFME and HS-SPME

Abstract

The essential oils and volatiles from the aerial parts of Salvia leriifolia obtained by hydrodistillation (HD), solvent-free microwave extraction (SFME) and headspace solid-phase microextraction (HS-SPME) approaches were analyzed using the GC and GC-MS instrumentations. Using the HD, SFME and HS-SPME methods, we were able to recognize thirty five, twenty seven and thirty seven components covering 95.30%, 98.35% and 93.32% of the oil compositions and volatile fractions, respectively. In the oil obtained by the HD method, β-pinene (13.88%), 1,8-cineole (13.74%), α-pinene (10.40%), α-cadinol (10.00%) and αbisabolol (7.35%) were the most abundant components whereas in the oil separated by the SFME approach, 1,8-cineole (24.13%), α-pinene (18.08%), β-pinene (11.91%), borneol (6.27%), α-cadinol (5.57%) and δcadinene (5.08%) were the major compounds. Also, in the volatile compounds isolated by the HS-SPME method, β-pinene (13.89%), 1,8-cineole (10.39%), α-pinene (8.74%) and δ-cadinene (8.25%) were the main fractions.