Chemical Composition of the Essential Oil from Flowers, Leaves and Stems of Haplophyllum perforatum by Using Head Space Solid Phase Microextraction

Abstract

The head-space volatiles separated from flowers, leaves and stems of Haplophyllum perforatum, a growing wild plant collected in the Golestan Province of Iran were investigated. A 100 μm polydimethylsiloxane (PDMS) fiber was conditioned to trap the volatiles prior to GC and GC-MS analyses. Sabinene (52.7 %, 24.7 %, and 26.2 %) and trans-carryophyllene (10.8 %, 35.6 %, and 8.8 %) were the main constituents in the flower, leaf, and stem oils of H. perforatum, respectively. The other components in the flower oil of the plant were (2E,6E)-farnesyl aceton (10.3 %), hexadecanoic acid (5.1 %), β-pinene (5.0 %) and cis-sabinene hydrate (4.9 %). Moreover, elemol (17.4 %), α-humulene (4.6 %), α-pinene (4.5 %) and 1,8-cineol (4.3 %) were found in the leave oil as well as camphor (7.4 %), limonene (6.3 %), elemol (5.0 %), β-phellandrene (4.9 %) and α-pinene (4.6 %) in the stem oil. The oils of flower and stem were consisted mainly of monoterpene hydrocarbons while the leave oil was characterized by sesquiterpene hydrocarbons as the main constituents.