Chemical composition of Oidiodendron kalrai.

Abstract

The chemical composition of yeast cells of Oidiodendron kalrai was analyzed and is expressed as percent dry weight. Cultures were grown in tryptone broth and in a liquid synthetic medium containing ammonium salts as a nitrogen source. After 48 h, carbohydrate levels were higher in the synthetic medium, but lipid levels were lower, Deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and protein contents did not differ significantly in the two media. The chemical components were also studied at different stages of growth. DNA remained relatively constant, but other components varied with the age of culture. The RNA was 6.6% at 18 h and declined rapidly to 5% by 24 h and remained constant, An initial protein content of 23% at 18 h increased rapidly to 37% by 48 h and gradually declined to 30% by day 10. The lipid content of 33% at 18 h decreased over the entire growth period to 10% by day 10. An initial carbohydrate level of 30% increased to a maximum of 54% by day 5 and then declined.