Abstract
The main pigment composition of Anemone obtusiloba was analysied using UV-vis spectrophotometry, HPLC and LC-MS. The results showed that there were two kinds of compounds, namely, carotenoids and flavonoids in the pigment, and the obsorbance of them regularly changed with the depth of flower color. From the HPLC and LC-MS analysis, seven kinds of flavonoids were found including luteolin-3-7-O-glucoside, quercetin-3-O-rutoside, quercetin-3-O-galactoside, quercetin-3-O-glucoside, quercetin-3-O-rhamnoside, kaempferol-3-O-sophoroside and myricetin-3-O-rhamnose and the derivative of quercetin was the main component in Anemone obtusifolia. From the flavonoid metabolism pathway, it could be determined that flavonoid 3'-hydroxylase (F3′H) was the key enzyme to increase the content of quercetin.
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