Abstract
ABSTRACT This study aimed to investigate the chemical composition and antifungal activities of essential oil (EO) of Teucrium luteum subsp. flavovirens. The analysis of EO using gas chromatography (GC-FID) and gas chromatography-mass spectroscopy (GC-MS) allowed the identification of 63 compounds, accounting for 89.9% of the total oil. The major constituents were E-nerolidol (31.0%), β-pinene (6.9%), elemol (5.0%), and α-pinene (4.5%). The antifungal activities of EO in liquid and vapor-phase were evaluated in vitro against three apple pathogenic fungi using poisoned food (PF) and volatile activity (VA) techniques. The results indicated that the EO inhibited significantly the mycelial growth of all strains tested (p < .05). The minimum inhibitory concentration (MIC) against Alternaria sp. was 2 µL/mL in VA assay, whereas >2 µL/mL in PF technique for all strains. Fungal spore production, in vapor phase, was completely inhibited at 2 µL/mL for three phytopathogens. The antifungal activities of T. luteum subsp. flavovirens from the Southeast of Morocco are reported for the first time.
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