Abstract
This work reports the chemical characterization, antioxidant and antimicrobial activity of Campomanesia xanthocarpa seed extracts obtained from compressed n-butane and supercritical CO2 extraction. For supercritical CO2, the extraction condition was 40°C and 250bar, while for n-butane 35°C and 10bar. The total phenolic compound content was 68.58±1.15mgg−1 for n-butane extract and 17.18±2.44mgg−1 for supercritical CO2. Both extracts were rich in terpenoids, especially β-caryophyllene, while n-butane extract had a larger content of flavonoids than supercritical CO2. The antioxidant activity was tested with FRAP, DPPH and deoxyribose assay, with n-butane extracts exhibiting better results. Antimicrobial activity was verified for both extracts against Pseudomonas aeruginosa, however, supercritical CO2 extract afforded a better performance, reaching 5.0mgmL−1 for MIC and 10.0mgmL−1 for MBC, while n-butane extract showed 40.0mgmL−1 in both antimicrobial tests. Higher extraction yields were obtained using n-butane, which also presented higher antioxidant activity, while supercritical CO2 extracts afforded the highest antimicrobial activity.
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