Chemical Composition, Antioxidant Activity, Cholinesterase Inhibitor and in Vitro Insecticidal Potentiality of Essential Oils of Lippia multiflora Moldenke and Eucalyptus globulus Labill. on the Main Carpophagous Pests of Cotton Plant in Ivory Coast.

Abstract

The abusive and repeated use of synthetic chemical insecticides has proven to be harmful to human health and the viability of the cotton production system in Ivory Coast, so it is imperative to find alternatives. . Thus, the objective of this study was to study the chemical composition and biological activity of essential oils of Lippia multiflora (Verbenaceae) and Eucalyptus globulus (Myrtaceae) and to evaluate their insecticidal potential in the laboratory on three main pests of cotton. After essential oils extraction, their chemical composition was determined. Also, antioxidant activity and cholinesterase inhibitor of essential oils were evaluated. After that, different concentrations of the two essential oils were prepared and applied by contact on groups of insects constituted by ten. The essential oil of L. multiflora was the most toxic for the three pests tested. Indeed, the lethal concentrations (LC50 ) were 1.74 %, 1.39 and 7.20 %, respectively, on Pectinophora gossypiella, Thaumatotibia leucotreta and Helicoverpa armigera. In contrast, the values obtained with E. globulus essential oil were nine to two times greater (16.05 %, 10.23 % and 16.32 %, respectively on these pests). With respect to the chemical composition of the essential oils, E. globulus essential oil was the richest in oxygenated monoterpenes (65 %) with 1,8-cineole or eucalyptol as the majority compound (61.6 %). The essential oil of L. multiflora was distinguished by a lower proportion of oxygenated monoterpenes (44.3 %), but it contained more terpene elements (24 vs. 15 for the essential oil of E. globulus). The essential oils of L. multiflora and E. globulus also showed significant inhibition of acetyl (2.13 and 2.16 mg galantamine equivalent (GALAE)/g, respectively) and butyryl cholinesterase (4.03 and 3.61 mg GALAE, respectively). L. multiflora was differentiated by its good inactivation of tyrosinases (163.46 versus 58.95 mg kojic acid equivalent (KAE)/g in E. globulus). Better antioxidant activity was observed with L. multiflora essential oil relative to DPPH (7.05±0.34 mg trolox equivalent (TE)/g). Biopesticides based on L. multiflora essential oil could be developed for the phytosanitary protection of cotton plant.