Abstract
BackgroundCupressus sempervirens is a medicinal plant traditional, its dried leaves are used in treatment of stomach pain, diabetes, inflammation, toothache, laryngitis and as contraceptive.MethodsThe present study was conducted to evaluate the in vitro antimicrobial, antibiofilm and determination chemical contents of the essential oil (Eo) and methanol extract from Mediterranean C. sempervirens L. The chemical composition of a hydrodistilled Eo of C. sempervirens was analyzed by a GC and GC/MS system.ResultsA total of 20 constituents representing 98.1% of the oil were identified: α-pinene (48.6%), δ-3-carene (22.1%), limonene (4.6%) and α-terpinolene (4.5%) were the main components comprising 79.8% of the oil. The antimicrobial test results showed that the methanol extract of C. sempervirens strongly inhibited the growth of the test bacteria studied, except for yeast species while the Eo had moderate antibacterial, but no anti-candida activity. Klebsiella pneumoniae was proven to be the most susceptible against methanol extract. The exposure time of Eo and methanol extract for complete inhibition of cell viability of K. pneumoniae was found to be 250 μg at 30 min and 500 μg at 120 min, respectively. The antibiofilm potential of the samples was evaluated using methods of PVC microtiter and eradication on biomaterial. Visual results showed visible biofilm eradication from the surface of intravenous infusion tube at 500 μg of Eo and methanol extract.ConclusionsThe results presented here may suggest that the Eo and extracts of C. sempervirens possess antimicrobial and antibiofilm properties, and therefore, can be used as natural preservative ingredients in food and/or pharmaceuticals.
Highlights
Cupressus sempervirens is a medicinal plant traditional, its dried leaves are used in treatment of stomach pain, diabetes, inflammation, toothache, laryngitis and as contraceptive
To the best of our knowledge, there are many papers report on the chemical composition of essential oils of C. sempervirens grown in north Mediterranean basin [6,7,8]
The aim of this work was to assay the main constituent of the essential oil obtained from leaves of Mediterranean C. sempervirens, and to carry out a comparative evaluation of their antibacterial, antibiofilm activities and chemical contents
Summary
Cupressus sempervirens is a medicinal plant traditional, its dried leaves are used in treatment of stomach pain, diabetes, inflammation, toothache, laryngitis and as contraceptive. C. sempervirens is a considered to be a medicinal tree, as its dried leaves are used for stomach pain, as well as to treat diabetes, and its dried fruit is used to treat inflammation, The geographic area of Cupressus genus is limited to the northern hemisphere and many species have been studied [5]. To the best of our knowledge, there are many papers report on the chemical composition of essential oils of C. sempervirens grown in north Mediterranean basin [6,7,8]. The aim of this work was to assay the main constituent of the essential oil obtained from leaves of Mediterranean C. sempervirens, and to carry out a comparative evaluation of their antibacterial, antibiofilm activities and chemical contents
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