Chemical Composition, Antifungal, and Cytotoxicity Activities of Inga laurina (Sw.) Willd Leaves.

Carla De Moura Martins,Luís F Leandro,Cláudio V Da Silva,Sérgio A L De Morais,Carlos H G Martins,Francisco J T De Aquino,Luís C S Cunha,Evandro A Do Nascimento,Mário M Martins,Roberto Chang,Alberto De Oliveira

doi:10.1155/2019/9423658

Abstract

The species Inga laurina is native to the Brazilian Cerrado. There are no studies about the chemical composition and biological activities of extracts of this endangered species. The ethanolic extract and its successive fractions are rich in phenolic compounds and presented good antifungal activities. HPLC/MS-MS/MS and H1/C13 analysis led to the identification of seventeen compounds, most of which are gallic acid derivatives, myricetin and quercetin glycosides. The ethyl acetate fraction (EAF) contained high levels of total phenolics, expressed in milligrams of gallic acid equivalents per gram of extract (475.3 ± 1.9 mg GAE gextract−1) and flavonoids expressed in milligrams of quercetin equivalents per gram of extract (359.3 ± 10.6 mg QE gextract−1). This fraction was active against fungi of the Candida genus. The EAF showed MIC value 11.7 μg mL−1 against C. glabrata and a selectivity index of 1.6 against Vero cells. The flavonol glycoside myricetin-3-O-rhamnoside was isolated for the first time from the Inga laurina. These results make I. laurina a promising plant as a source of pharmaceutical and biological active antifungal compounds.

Highlights

Among the plants in danger of extinction from Brazilian savannah biome (Cerrado), the Inga spp. (Fabaceae) are found mainly in neotropical areas and many plants from this family have been reported to possess biological activities and several are used in folk medicine [1]
The present work is based on a doctorate thesis and the aim of the present work was to identify the bioactive metabolites by Mass Spectrometry and to evaluate the antifungal activity against Candida genus and the cytotoxic activity on Vero cells ATCC CCL 81 using ethanolic extract and fractions of I. laurina leaves
ethanolic extract (EE), ethyl acetate fraction (EAF), and BF fractions presented the highest contents of total phenolics

Summary

Introduction

Among the plants in danger of extinction from Brazilian savannah biome (Cerrado), the Inga spp. (Fabaceae) are found mainly in neotropical areas and many plants from this family have been reported to possess biological activities and several are used in folk medicine [1]. Several biological activities have been observed in Inga species, such as the antioxidant activity of I. edulis [4,5,6] and I. verna [2], the antifungal activity of I. marginata [7], the antimicrobial activity of I. fendleriana [8], the allelochemical effects in I. umbellifera [9], and the antitumoral activity in I. marginata [10]. I. laurina is found in the Cerrado biome but in different regions of Brazil: Amazon, Caatinga, and the Atlantic Coast. This plant is found in Brazil, but there are a few studies about its essential oil. The present work is based on a doctorate thesis and the aim of the present work was to identify the bioactive metabolites by Mass Spectrometry and to evaluate the antifungal activity against Candida genus and the cytotoxic activity on Vero cells ATCC CCL 81 using ethanolic extract and fractions of I. laurina leaves

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Results

Conclusion