Chemical Composition and Protective Effect of Young Barley (Hordeum vulgare L.) Dietary Supplements Extracts on UV-Treated Human Skin Fibroblasts in In Vitro Studies.

Krystyna Joanna Gromkowska-Kępka,Renata Markiewicz-Żukowska,Katarzyna Socha,Justyna Moskwa,Anna Puścion-Jakubik,Joanna Bielecka,Sylwia Katarzyna Naliwajko,Jolanta Soroczyńska,Monika Grabia,Konrad Mielcarek,Patryk Nowakowski

doi:10.3390/antiox10091402

Krystyna Joanna Gromkowska-Kępka, Renata Markiewicz-Żukowska + Show 9 more

Open Access

https://doi.org/10.3390/antiox10091402

Copy DOI

Abstract

Young barley seems to be a promising material for use as nutricosmetic due to the presence of many biologically active compounds. The aim of this study was to evaluate the effect of Hordeum vulgare L. extracts on human skin fibroblasts exposed to ultraviolet radiation B (UVB) radiation. Analysis of the chemical composition showed a predominance of 9,12,15-octadecatrienoic acid. The quality assessment showed that young barley preparations have high total polyphenolic content (TPC) and favourable total antioxidant status (TAS). They also contain antioxidant elements such as zinc, copper, and selenium. Furthermore, the analyzed products were found to be safe in terms of toxic elements (lead, cadmium and mercury) and lack of cytotoxic effect of young barley extracts on cells. In vitro bioactivity assays showed that young barley extract increased the survival rate and accelerated the migration of fibroblasts in research models with UVB radiation. The application of both extracts caused an increase in DNA biosynthesis, and in the number of cells arrested in S phase. Moreover, an inhibitory effect of the tested extracts on the expression of matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9) was observed. The results indicate that young barley extracts, due to protective as well as restorative effect, could potentially be used in the production of nutricosmetics and skin care products.

Highlights

IntroductionAs the very sensitive and most external organ, is constantly exposed to damaging factors; it is the most vulnerable to anatomical and functional changes associated with aging
Skin, as the very sensitive and most external organ, is constantly exposed to damaging factors; it is the most vulnerable to anatomical and functional changes associated with aging.The process of skin aging is inseparably connected with changes in physical, morphological and physiological properties of individual skin layers
The young barley products selected for the study, which were commercially available as dietary supplements, did not have information on the label regarding zinc, copper, or selenium content

Summary

Introduction

As the very sensitive and most external organ, is constantly exposed to damaging factors; it is the most vulnerable to anatomical and functional changes associated with aging. The process of skin aging is inseparably connected with changes in physical, morphological and physiological properties of individual skin layers. The essence of the aging process is changes in the histological image of the epidermis and dermis. The modifications stimulated by environmental (exogenous) conditions are superimposed on the changes occurring as a result of genetically determined modifications (chronological ageing) [1,2]. Using sunscreens reduces the amount of UV to which the skin is exposed and reduces the occurrence of erythema and other changes leading to photoaging

Objectives

Methods

Discussion

Conclusion