Abstract
BackgroundConsumption of vegetables has been proven to be effective in the prevention of different diseases. Traditionally edible aerial part of Pisum sativum L. subsp. sativum (Fabaceae) is used to treat diabetes, heart diseases and as blood purifier. Present study was aimed to explore the traditional use of aerial parts of P. sativum as a source of antidiabetic agent. In addition, antioxidant activity and chemical composition was carried out.MethodsTotal polyphenol content was spectrophotometrically determined using Folin Chiocalteu’s reagent while the flavonoids by aluminum chloride colorimetric assay. Identification of compounds of the extract was made through HPLC and LCMS. Antihyperglycemic activity was assessed by oral glucose tolerance test in mice. Antioxidant activity was determined by DPPH free radical scavenging and reducing power assay.ResultsTotal polyphenol and total flavonoids content were found to be 51.23 mg gallic acid equivalent and 30.88 mg quercetin equivalent per gram of dried plant extract respectively. Ellagic acid and p-coumeric acid were detected through HPLC. A total of eight compounds including naringenin, β-sitosterol were indentified through LCMS. In OGTT, extract (200 mg/kg bw) showed a 30.24% decrease (P< 0.05) in blood glucose levels at 30 min compared to the normal control. The extract showed IC50 value of 158.52 μg/mL in DPPH scavenging assay and also showed comparable reducing power.ConclusionAlong with other compounds ellagic acid and β-sitosterol present in the extract may be responsible for its antioxidant as well as antihyperglycemic activities. Altogether these results rationalize the use of this vegetable in traditional medicine.
Highlights
Consumption of vegetables has been proven to be effective in the prevention of different diseases
Glucose oxidation is believed to be the main source of these free radicals that are responsible for destruction of the pancreatic beta cells responsible for producing insulin [2]
Quercetin as standard was used to obtain a calibration curve with the equation y = 1.169+ 0.008, Coefficient of Determination (R2) = 0.994 from which total flavonoid content of the extract was calculated as Polyphenolic Compound Content % RSDa p-Coumaric acid
Summary
Consumption of vegetables has been proven to be effective in the prevention of different diseases. Sativum (Fabaceae) is used to treat diabetes, heart diseases and as blood purifier. Glucose oxidation is believed to be the main source of these free radicals that are responsible for destruction of the pancreatic beta cells responsible for producing insulin [2] Are these radicals involved in the cause of diabetes, they appear to play a role in some of the complications. Antioxidant, antimicrobial and hypoglycemic activities of seeds have been reported [9]. Growth tender (leaves and stem) is used as vegetables in Bangladesh [14] It is traditionally used in treatment of diabetes, heart diseases and as blood purifier [14]
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