Chemical composition and molecular structure of polysaccharide-protein biopolymer from Durio zibethinusseed: extraction and purification process

Bahareh Tabatabaee Amid,Sanja Kostadinović,Hamed Mirhosseini

doi:10.1186/1752-153x-6-117

Abstract

BackgroundThe biological functions of natural biopolymers from plant sources depend on their chemical composition and molecular structure. In addition, the extraction and further processing conditions significantly influence the chemical and molecular structure of the plant biopolymer. The main objective of the present study was to characterize the chemical and molecular structure of a natural biopolymer from Durio zibethinus seed. A size-exclusion chromatography coupled to multi angle laser light-scattering (SEC-MALS) was applied to analyze the molecular weight (Mw), number average molecular weight (Mn), and polydispersity index (Mw/Mn).ResultsThe most abundant monosaccharide in the carbohydrate composition of durian seed gum were galactose (48.6-59.9%), glucose (37.1-45.1%), arabinose (0.58-3.41%), and xylose (0.3-3.21%). The predominant fatty acid of the lipid fraction from the durian seed gum were palmitic acid (C16:0), palmitoleic acid (C16:1), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), and linolenic acid (C18:2). The most abundant amino acids of durian seed gum were: leucine (30.9-37.3%), lysine (6.04-8.36%), aspartic acid (6.10-7.19%), glycine (6.07-7.42%), alanine (5.24-6.14%), glutamic acid (5.57-7.09%), valine (4.5-5.50%), proline (3.87-4.81%), serine (4.39-5.18%), threonine (3.44-6.50%), isoleucine (3.30-4.07%), and phenylalanine (3.11-9.04%).ConclusionThe presence of essential amino acids in the chemical structure of durian seed gum reinforces its nutritional value.

Highlights

The biological functions of natural biopolymers from plant sources depend on their chemical composition and molecular structure
The results demonstrated the presence of a high quantity of glucose (40.8-44.6%) in the carbohydrate profile of durian seed gum
As reported by Palanuvej et al [8], galactose and glucose were the main monosaccharide in the chemical composition of glucomannan from Litsea glutinosa leaves, Hibiscus esculentus and Scaphium scaphigerum fruits, Ocimum canum, Plantago ovata and Trigonella foenum-graecum seeds

Summary

Introduction

The biological functions of natural biopolymers from plant sources depend on their chemical composition and molecular structure. The main objective of the present study was to characterize the chemical and molecular structure of a natural biopolymer from Durio zibethinus seed. The botanical taxonomy of durian brings to light many of the taxonomic problems. The genus Durio was created by Rumphius (1741) in his ‘Herbarium Amboinense’. Later, it was rendered into Linnaean by Adanson [3]. Some researchers attributed the species to Linnaeus (L.) which appeared several times in early taxonomic literature. The earliest valid publication (Murray, 1774) indicates that the botanical taxonomy of the species is referred to ‘D. zibethinus Linnaeus (L.). Willdenow (1800) listed ‘Durio zibethinus as the species to Linnaeus (L.) [2,3]

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Results

Conclusion