Abstract

The organic matrix accounts for 2 to 3% of the total stone weight. Since the stone matrix consists of many distinct proteins, the chemical properties and the functional role should be examined for each matrix protein. Some previous studies have fractionated the matrix proteins using conventional chromatography. However, it this time-consuming and the resolution ability leaves much to be desired. The aim of the present study is to investigate the protein composition and the chemical properties of the matrix in relation to the mineral composition, using high-performance gel permeation liquid chromatography (HPLC-GPC) and high-performance ion-exchange liquid chromatography (HPLC-IEX).

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