Abstract
Purpose: To investigate the chemical composition and bioactivity of the acetone extract of Pleurosticta acetabulum lichen. Methods: Phytochemical analysis of the acetone extract of the lichen (Pleurosticta acetabulum) was carried out by high-performance liquid chromatography (HPLC). The antioxidant activity of the lichen extract was evaluated by determining the radical scavenging capacity on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and reducing power. To determine total phenolics and flavonoids, we used spectrophotometric methods. Antimicrobial activity was estimated by determination of the minimal inhibitory concentration using broth microdilution method. Anticancer activity of the lichen extract was tested using 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT). Results: Salazinic, norstictic, protocetraric, evernic acid and atranorin were identified as compounds of lichen. P. acetabulum extract exhibited moderate free radical scavenging activity (half-maximum inhibitory concentration, IC50 of 151.7301 µg/mL). The spectrophotomeric absorbance of the extract for reducing power varied from 0.035 to 0.127, while the total phenolics and flavonoids in the extract were 35.39 µg PE/mg and 12.74 μg RE/mg, respectively. Minimum inhibitory concentration (MIC) was in the range of 1.25 to 20 mg/mL while cytotoxic activity (based on IC50 values) ranged from 24.09 to 45.94 µg/mL. Conclusion: The results confirm that lichen extract contains secondary metabolites that possess antioxidant, antimicrobial and anticancer activities, which opens up some possibilities for the extract to be developed as food supplements and pharmaceutical raw materials. Keywords: Pleurosticta acetabulum, Antioxidant activity, Antimicrobial activity, Cytotoxic activity, Lichen
Highlights
A great diversity of living organisms can be used for the improvement of human health, such as biologically active components extracted from the plants, mushrooms and lichens [1,2,3]
The aim of this study is to present the results of the phytochemical analysis of the acetone extract of P. acetabulum lichen and its antioxidant, antimicrobial and anticancer activities in order to search for available natural antioxidant, antibiotic and anticancer agents that can be used as possible food supplements, raw materials for the pharmaceutical industry and remedies for the treatment of various diseases
The chromatograms for standards and lichen acetone extract eluted by high-performance liquid chromatography (HPLC) are represented in Figure 1 and Figure 2
Summary
A great diversity of living organisms can be used for the improvement of human health, such as biologically active components extracted from the plants, mushrooms and lichens [1,2,3]. In the literature, only a few studies have investigated the potential of Pleurosticta acetabulum lichen. The aim of this study is to present the results of the phytochemical analysis of the acetone extract of P. acetabulum lichen and its antioxidant, antimicrobial and anticancer activities in order to search for available natural antioxidant, antibiotic and anticancer agents that can be used as possible food supplements, raw materials for the pharmaceutical industry and remedies for the treatment of various diseases. The samples of lichen P. acetabulum (Neck.) Elix & Lumbsch were collected from the mountain Kopaonik, Serbia, during May 2013. The samples of collected species of lichen have been herbariumstored at the Department of Biology and Ecology of Kragujevac, Faculty of Science,
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
