Chemical composition and antiparasitic activity of essential oils from leaves of Guatteria friesiana and Guatteria pogonopus (Annonaceae)

Cássio S Meira,Leociley R A Menezes,Tatiana B Dos Santos,Taís S Macedo,José E N Fontes,Emmanoel V Costa,Maria L B Pinheiro,Thanany B Da Silva,Elisalva Teixeira Guimarães,Milena B P Soares

doi:10.1080/10412905.2016.1210041

Abstract

Natural products represent a valuable source for discovery of antiparasitic agents. Here, we describe the antiparasitic activity from essential oils extracted from leaves of Guatteria friesiana (EOGF) and Guatteria pogonopus (EOGP) (Annonaceae). The essential oils were obtained by hydrodistillation and analyzed by GC/MS and GC-FID. The sesquiterpenes are more abundant in both essential oils. G. friesiana are dominated by β-eudesmol (51.9%), γ-eudesmol (18.9%) andα-eudesmol (12.6%). The major compounds identified for EOGP were spathulenol (24.8%), γ-amorphene (14.7%) and germacrene D (11.8%). The essential oils demonstrated potent trypanocidal and antimalarial activities with values of IC50 lower than 41.3 μg/mL. EOGF also inhibits the proliferation of amastigotes. In addition, we identified significant ultrastructural alterations induced by the essential oils, especially in the cell membrane, Golgi complex, endoplasmatic reticulum and mitochondria. The results presented herein reinforce the potential of other members of this family for search of antiparasitic compounds.

Highlights

Chagas disease and malaria are caused by protozoan parasites and constitute serious public health problems worldwide, mainly in tropical countries [1]
Antimalarial activity Parasites grown at 1–2% parasitemia and 2.5% hematocrit were plated onto 96-wells plate and incubated with the compounds at different concentrations ranging from 1.23 μg/mL to 100 μg/mL in culture medium (RPMI 1640)
Bloodstream trypomastigotes forms of T. cruzi were cultured in 96-well plates (4 × 105 cell/well) in RPMI medium supplemented with 10% fetal bovine serum (FBS) and 50 μg/mL of gentamycin in the absence or presence of different concentrations of the essential oils, in triplicate

Summary

Introduction

Chagas disease and malaria are caused by protozoan parasites and constitute serious public health problems worldwide, mainly in tropical countries [1]. Previous phytochemical studies on these species described the isolation of alkaloids, as well as, yours cytotoxic activities and the composition of the essential oil [15, 16]. In the continuous search for antiparasitic compounds from Brazilian Annonaceous plants, the chemical composition and antiparasitic activity of the essential oils

Results

Conclusion