Chemical composition and antioxidant activity of beebread, and its influence on the glioblastoma cell line (U87MG)

Renata Markiewicz-Żukowska,Valery Isidorov,Justyna Moskwa,Jolanta Soroczyńska,Sylwia K Naliwajko,Emilia Bartosiuk,Maria H Borawska

doi:10.2478/jas-2013-0025

Renata Markiewicz-Żukowska, Valery Isidorov + Show 5 more

Open Access

https://doi.org/10.2478/jas-2013-0025

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Abstract

Abstract Beebread is processed pollen stored in the cells of the honeycomb, with the addition of various enzymes and honey, which undergoes lactic acid fermentation. Ethanolic extracts (EBBs) were obtained from three different samples of beebread from Poland. Assays were carried out for the determination of chemical composition (GC/MS), for the total phenolic content, and for the antioxidant and cytotoxic activities. The effects of beebread extracts (10, 20, 30, 50, 100 μg/mL) on the viability of the glioblastoma cell line (U87MG) were studied after 24 h, 48 h, and 72 h. Our results indicated a time-dependent inhibitory effect on the viability of U87MG cells treated EBB. The main inhibitory effect of EBB was observed after 72 h; EBB treatment decreased cell viability to 49 - 66%.

Highlights

Beebread is a fermented mixture of plant pollen, honey, and bee saliva that worker bees use as food for the larvae, and for young bees to produce royal jelly
Studies assessing the efficacy of treatments with beekeeping products for patients with atherogenic dyslipidemia showed that a significant hypolipidemic effect was registered in patients taking honey in combination with beebread (Kas’ianenko et al, 2011)
There are 64 compounds, 37 of which are registered in all three beebread samples

Summary

Introduction

Beebread is a fermented mixture of plant pollen, honey, and bee saliva that worker bees use as food for the larvae, and for young bees to produce royal jelly. Pollen collected by bees is mixed with a small amount of honey and saliva and packed into the cells of the honeycomb where it undergoes a chemical change to form a product called beebread (Gilliam, 1979). Beebread has a positive effect on the immune system of healthy people. It has antibiotic and antioxidant properties (Audisio et al, 2005; Mutsaers et al, 2005; Baltrušaitytė et al, 2007a). Studies assessing the efficacy of treatments with beekeeping products for patients with atherogenic dyslipidemia showed that a significant hypolipidemic effect was registered in patients taking honey in combination with beebread (total cholesterol decreased by 15.7%, LDL cholesterol by 20.5%) (Kas’ianenko et al, 2011). The anticancer activity of propolis and honey have been widely

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