Abstract
Purpose: To evaluate the effect of the essential oil of Origanum vulgare Linnaeus (Lamiaceae) on the growth of Sporothrix schenckii and Sporothrix brasiliensis.Methods: The chemical composition of the essential oil was investigated by gas chromatography/flame ionization detector (GC-FID). The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were determined by broth micro-dilution method. Scanning electron microscopy (SEM) was also performed to reveal morphological alterations in Sporothrix spp. cells.Results: The major components of the essential oil were γ-terpinene (30.5%), carvacrol (15.7 %) and 4-terpineol (13.0 %). γ-Terpinene showed potential antifungal activity with MIC ranging from 62.5 to 500.0 μg mL-1 for S. schenckii, and 125.0 to 250.0 μg mL-1 for S. brasiliensis. SEM micrographs revealed morphological alterations in hyphae and reduction of the adhered conidia numbers.Conclusion: Origanum vulgare Linnaeus essential oil possesses potential antifungal activity, and can, therefore, can be developed as an alternative agent for the treatment of sporotrichosis.Keywords: Antifungal Activity, Essential Oil, Gas Chromatography, Origanum vulgare, Sporotrichosis
Highlights
Sporotrichosis is a subcutaneous mycosis affecting humans and animals, with worldwide distribution, especially in tropical and subtropical areas, constituting an important public health problem [1]
Chemical composition of the essential oil Seventeen compounds were identified in O. vulgare essential oil, accounting for 91.6 % of the whole composition (Table 1)
The results of the microbiological screening revealed that the O. vulgare essential oil had inhibitory activity against the tested fungal species
Summary
Sporotrichosis is a subcutaneous mycosis affecting humans and animals, with worldwide distribution, especially in tropical and subtropical areas, constituting an important public health problem [1]. There may be secondary transmission to the bones, joints and muscles [2] It is caused by fungus of the Sporothrix schenckii complex, being Sporothrix sckenckii and Sporothrix brasiliensis the species more frequently related in clinical samples of Brazil [3]. Its clinical uses have been limited by the emergence of drug resistance, high risk of toxicity, insufficiencies in their antifungal activity and undesirable side effects [5]. Considering these factors, there is a need for the discovery of new agents with antifungal potential
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